Joy Cheek had an offensive rebound and put layup with 1.2 seconds to win the game for Duke.
Duke broke a two-game streak of being outrebounded as the Blue Devils held a 42-37 advantage on the boards.
Duke had a season-high 25 turnovers.
The Blue Devils improve to 2-0 in the all-time series with Pittsburgh.
Duke blocked 10 shots for the second straight game. The Blue Devils have swatted 10 or more shots three times this season.
Senior Emily Waner played seven minutes and hit a key three-pointer with 6:31 remaining to put the Blue Devils ahead by seven points.
Sophomore Joy Cheek scored 13 points in the second half as she finished the game with a season-high 16 points. She scored double-figures for the fourth time this season and the first since the third game of the year against UNC Greensboro on Nov. 18.
Junior Abby Waner scored 10 points, which was her third game this season with double-figure points. She also had four rebounds, three assists and three steals.
Sophomore Keturah Jackson blocked a career-high two shots in 17 minutes.
Duke continued to open with slow starts on the season as the Blue Devils were down 10-4 at the 15:35 mark.
The Blue Devils played their second straight BIG EAST opponent and improved to 3-1 against the BIG EAST this season.
Chante Black broke a string of eight straight games scoring double-figures as she finished the afternoon with seven points, four rebounds, a season-high four assists, six blocks and one steal in 27 minutes.
Coming off blocking a career-high six blocks against Rutgers on Thursday, junior Chante Black swatted five shots in the first half and finished the game with six.
Duke's 24 first half points was tied for the second-lowest of the season in the first stanza.
The Blue Devils were tied 24-24 at the half marking the first time this season a score has been tied after 20 minutes.
Duke defeated Pitt Head Coach Agnus Berenato for the 23rd straight time (2 at Pitt and 21 at Georgia Tech)
For the second time this season, Duke kicked off the game with the same starting lineup in back-to-back games. On Saturday, Jasmine Thomas, Abby Waner, Bridgette Mitchell, Joy Cheek and Chante Black started the contest. Duke has started eight different starting lineups this season in 10 games.
Junior Carrem Gay played in her homecoming game on Saturday as she is from New York, N.Y., and attended Christ The King H.S. In the contest, she played 23 minutes and had five points, four rebounds, two assists, two blocks and one steal.
Duke will next have nine days off for exams before hosting Utah Valley on Dec. 18 at 7:00 PM in Cameron Indoor Stadium.